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| CYT-398 | 重組豬白介素-4（rPIL-4） | 2μg/10μg/1mg | Prospec |
| Background : | |
| Interleukin-4 （IL4） has a compact, globular fold （similar to other cytokines）, stabilised by 3 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. Interleukin （IL）-4, produced primarily by CD4（+） T cells is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin （IL）-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses. | |
 | Description : |
| Recombinant Porcine IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12615 Dalton. Recombinant Porcine IL-4 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| Lyophilized from a concentrated （1mg/ml） solution in water containing no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Porcine IL-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Recombinant Porcine IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution, IL-4 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Porcine IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is < 10 ng/ml, corresponding to a Specific Activity of 1 x 105 IU/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Porcine IL-4. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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