The Thermo Scientific Pierce Classic IP Kit provides all the necessary reagents, spin cups and collection tubes to perform successful immunoprecipitation （IP） experiments with ease.

The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.

Highlights:

• Assay consistency – Pierce Spin Columns （see dimensions） eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
• Fast – Immunoprecipitate （IP） in less than one hour
• Mild elution conditions – Recover antigen without harsh detergents or reducing agents
• Easy-to-follow instructions – IP target protein from crude lysate in four simple steps
• Suitable for most common antibody types – Protein A/G provides excellent binding for most mouse, rabbit, human and goat IgG subclasses
• Complete kit – includes lysis/wash buffer, binding and elution buffers, control agarose resin, sample loading buffer and spin columns