Thermo Scientific Pierce Hydroxylamine-HCl has many uses, which includes cleaving Asn-Gly peptide bonds and deprotecting （exposing） sulfhydryls on SATA-modified molecules for conjugation.

Hydroxylamine•HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds （aldehydes and ketones） to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine•HCl.

Highlights:

• Exposes protected sulfhydryl groups on SATA-modified molecules
• Cleaves carbonyl-containing crosslinkers such as EGS