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| CYT-250 | 重組人白介素-17 （IL-17） | 5μg/25μg/1mg | Prospec |
| Description : | |
| Recombinant Human IL-17A produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids and having a molecular mass of 30,250 Dalton. Recombinant IL-17 is purified by proprietary chromatographic techniques. | |
 | Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| Lyophilized from a concentrated （1mg/ml） solution containing no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Human IL-17 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized Recombinant IL-17A although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-17 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ile-Thr-Ile-Pro. | |
| Biological Activity: |
| The ED50 as determined by the dose-dependant induction of IL-6 in primary human foreskin fibroblasts was found to be 2 ng/mL, corresponding to a specific activity of 5 x 105 Units/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Recombinant Human IL-17. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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