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| CYT-427 | 重組人前骨形態(tài)發(fā)生蛋白-2（rHuProBMP-2） | 2μg/10μg/100ug | Prospec |
| Description: | |
| Recombinant Human ProBMP-2 is the precursor of BMP-2 which has 3 intramolecular cystein bridges is a homodimer, non-glycosylated, Polypeptide chain containing 376 amino acids and having a molecular mass of 85,726 Dalton. Recombinant ProBMP-2 is purified by proprietary chromatographic techniques. | |
 | Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| The protein was lyophilized from a concentrated （0.1mg/ml） sterile solution containing 10mM HCL pH=2. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Recombinant Human ProBMP-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized ProBMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant ProBMP-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| GAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYR RHSGUPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQ VFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTRLLDTRLVNQNASRWESFDVTPAVMRW TAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDJKJHPLHKREK RQAHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAYVQ TLVNSVNSKIPKACCVPSAISMLYLDENKVVLKNYQDMVVEGCGCR | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| The ED50 as determined by the stimulating effect on the migration of C3T3 cells and was found active. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of ProBMP-2. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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