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| CYT-249 | 重組人血管生成素樣蛋白-4（rHuANGPTL4） | 2μg/10μg/1mg | Prospec |
| Background: | |
| The fasting-induced adipose factor （FIAF, ANGPTL4, PGAR, HFARP） was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, FIAF was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated FIAF, whereas in human white adipose tissue and SGBS adipocytes, only the native FIAF could be detected. Interestingly, the truncated FIAF was produced by human liver. Experimental data suggest that FIAF is mainly presented in human blood plasma in a truncated form （FIAF-S2）, whose level is increased by fenofibrate treatment. Levels of both truncated and native FIAF showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation. | |
 | Description: |
| Recombinant Human Angiopoietin-like Protein 4 manufactured with N-terminal HisTag, is a 25 kDa polypeptide containing 204 amino acid residues of the Human ANGPTL4 and 16 additional amino acid residues. | |
| Amino Acid sequence: |
| MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIL FHKVAQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR | |
| Physical Appearance: |
| Sterile Filtered lyophilized （freeze-dried） powder. | |
| Formulation: |
| Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4. | |
| Specificity: |
| The amino acid sequence of the recombinant Human ANGPTL4 is 100% homologous to the 26-229 amino acid sequence of the Human ANGPTL4 precursor without signal sequence. | |
| Purification Method: |
| One-step procedure using affinity Ni-NTA chromatography. | |
| Purity: |
| >95% （SDS-PAGE analyzed）. | |
| Reconstitution: |
| Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve compley. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. | |
| Stability/Shelf Life: |
| Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. | |
| Applications: |
| Western blotting, ELISA | |
| References: |
| • Mandard S, Zandbergen F, Tan NS, Escher P, Patsouris D, Koenig W, Kleemann R, Bakker A, Veenman F, Wahli W, Muller M, Kersten S.: The direct peroxisome proliferator-activated receptor target fasting-induced adipose factor FIAF/PGAR/ANGPTL4） is present in blood plasma as a truncated protein that is increased by fenofibrate treatment. J Biol Chem. 2004 Aug 13;279（33）:34411-20. Epub 2004 Jun 9. • Koster A, Chao YB, Mosior M, Ford A, Gonzalez-DeWhitt PA, Hale JE, Li D, Qiu Y, Fraser CC, Yang DD, Heuer JG, Jaskunas SR, Eacho P. Transgenic angiopoietin-like （angptl）4 overexpression and targeted disruption of angptl4 and angptl3: regulation of triglyceride metabolism. Endocrinology. 2005 Nov;146（11）:4943-50. Epub 2005 Aug 4. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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