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CYT-364

重組人酸性成纖維細(xì)胞生長因子（rHuFGF-a/gly）

2μg/10μg/1mg

Prospec

Background :
	Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor. Other homologous FGF belonging to the same family are int-2 （FGF-3 ）, FGF-5 , FGF-6 , K-FGF and KGF （keratinocyte growth factor =FGF-7 ）. All factors are products of different genes, some of which are Oncogene products （FGF-3 , FGF-4 , FGF-5 ）.
	Description :
	Recombinant Human FGF-a （FGF-1） produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton. Recombinant Human FGF-a is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered liquid formulation.
	Formulation:
	The sterile protein solution （1.8mg/ml） contains 20mM Tris HCl pH=7.9, 100mM KCl, 0.2mM DTT and 20% glycerol.
	Stability:
	Recombinant Human FGF-a although stable at 4⁰C for 3 weeks, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Asn-Leu-Pro.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s Recombinant Human FGF-a is fully biologically active when compared to standards. The ED₅₀, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors （measured by ³H-thymidine uptake） is <10 ng/ml, corresponding to a specific activity of 10⁵ Units/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of FGF-acidic-Sf9.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of FGF-a as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

佰易聚生物商城提供美國光譜醫(yī)學(xué)透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！

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