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| CYT-448 | 重組人Myostatin Propeptide（rHuGDF-8） | 5ug/25ug | Prospec |
| Introduction: | |
| Myostatin （GDF-8）, a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority （>70%） of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo. | |
 | Description: |
| 28 kDa protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional amino acid residues. MG NENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Specificity: |
| The amino acid sequence of the recombinant human Myostatin Propeptide is 100% homologous to the amino acid sequence of the human Myostatin Propeptide without signal sequence. | |
| Purification Method: |
| Two-step procedure using size exclusion chromatography before and after refolding. | |
| Purity: |
| >95% （SDS-PAGE analyzed）. | |
| Formulation: |
| Lyophilized from a concentrated （1mg/ml） solution containing 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 8.0. | |
| Solubility: |
| Add 0.2 ml of d H2O and let the lyophilized pellet dissolve compley. | |
| Stability: |
| Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20°C. | |
| Applications: |
| Western blotting, ELISA | |
| References: |
| • Jiang MS, Liang LF, Wang S, Ratovitski T, Holmstrom J, Barker C, Stotish R. Characterization and identification of the inhibitory domain of GDF-8 propeptide. Biochem Biophys Res Commun. 2004 Mar 12;315（3）:525-31. • Hill JJ, Davies MV, Pearson AA, Wang JH, Hewick RM, Wolfman NM, Qiu Y. The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum. J Biol Chem. 2002 Oct 25;277（43）:40735-41. Epub 2002 Aug 22. • Thies RS, Chen T, Davies MV, Tomkinson KN, Pearson AA, Shakey QA, Wolfman NM. GDF-8 propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF-8 receptor binding. Growth Factors. 2001;18（4）:251-9. • Lee SJ, McPherron AC. Regulation of myostatin activity and muscle growth. Proc Natl Acad Sci USA. 2001 Jul 31;98（16）:9306-11. Epub 2001 Jul 17. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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