Thermo Scientific Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein isothiocyanate (FITC) and NHS-Fluorescein are amine-reactive derivatives of fluorescein dye that have wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Fluorescein-5-maleimide and 5-IAF are sulfhydryl-reactive derivatives of fluorescein dye. Choose from stand-alone packages of these labeling reagents or select a convenient FITC or NHS-Fluorescein Antibody Labeling Kit.

Highlights:

• Fast – label and purify antibody in about one hour
• Amine-specific labeling – the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
• Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence
• Single-use fluors – no need to weigh tiny amounts powder; kits contain single-use vials of reagent
• Efficient purification – kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery