訂貨：，，

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA品牌介紹

美國Pierce公司（www.pierce.com）是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 主要產品包括：

1）化學發光體和比色底物

 2）分析試劑

 3）熒光標記探針

 4）酶聯免疫分析試劑盒等

（SULFO-SMCC）SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA用于科研

Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.

Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Sulfo-SMCC Highlights

• Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
• Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
• Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
• High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
• Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.

• 佰易聚生物商城提供優質的分子生物學試劑和試劑盒、美國聯合碳化和美國光譜醫學透析袋、培養基和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄www.ebioeasy.com